促甲狀腺素(TSH)檢測試劑盒(酶聯免疫吸附試驗法)

ELISA Kit for Thyroid Stimulating Hormone (TSH)

Thyrotropin

  • 促甲狀腺素(TSH)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 促甲狀腺素(TSH)檢測試劑盒(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 促甲狀腺素(TSH)檢測試劑盒(酶聯免疫吸附試驗法) 實驗結果圖
  • CEA463Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測促甲狀腺素(TSH),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的促甲狀腺素(TSH)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 91-105 95
EDTA plasma(n=5) 96-104 99
heparin plasma(n=5) 88-102 92

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的促甲狀腺素(TSH),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中促甲狀腺素(TSH)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 78-97% 95-105% 80-88% 97-105%
EDTA plasma(n=5) 78-96% 80-103% 84-97% 91-98%
heparin plasma(n=5) 94-102% 78-97% 80-102% 91-99%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)50µL,
    加入50µL檢測液A(臨用前配制);
    37°C溫育1小時。
3. 洗板3次;
4. 加檢測溶液B100µL,37°C孵育30分鐘;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分鐘;
7. 加終止液50µL,立即450nm讀數。

實驗原理

本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將促甲狀腺素(TSH)單克隆抗體包被微孔板,制成固相載體,往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本),待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物,然后加入HRP標記的親和素,經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。待測標本濃度越高,標記抗原和抗體的結合就越受到抑制,顯色愈淺。顯色的深淺與酶量呈正相關,而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
PAA463Mu01 促甲狀腺素(TSH)多克隆抗體 WB; IHC; ICC; IP.
CEA463Mu 促甲狀腺素(TSH)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA463Mu 促甲狀腺素(TSH)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA463Mu11 促甲狀腺素(TSH)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

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